TEMPO-derived spin labels linked to the nucleobases adenine and cytosine for probing local structural perturbations in DNA by EPR spectroscopy

• Dnyaneshwar B. Gophane and
• Snorri Th. Sigurdsson

Beilstein J. Org. Chem. 2015, 11, 219–227, doi:10.3762/bjoc.11.24

• labels; structure-dependent dynamics; Introduction The knowledge about structures and conformational dynamics of nucleic acids, as well as other biomolecules, is essential to understand their biological functions, including interactions with other molecules. The exact atom-to-atom structural information

• the investigation of single-base mismatches [51][54][56][58][59], abasic sites [60] as well as nick sites in duplex DNA [61], and ligand-induced folding of riboswitches [62][63]. Continuous wave (CW) EPR spectroscopy can be used for the determination of structure-dependent dynamics based on the line